Journal of Equine Veterinary Science
Volume 29, Issue 11 , Pages 795-798 , November 2009

A Comparison Between Polymerase Chain Reaction and Enzyme-Linked Immunosorbent Assay Methods for Detecting Leptospira in Equine Recurrent Uveitis

  • Gholam Ali Kojouri, DVM, PhD

      Affiliations

    • Department of Clinical Sciences, School of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
    • Corresponding Author InformationReprint requests: Gholam Ali Kojouri, Department of Clinical Sciences, School of Veterinary Medicine, Shahrekord University, PO Box 115, Shahrekord, Iran.
    • ,
  • Camellia Taghadosi, DVM

      Affiliations

    • Department of Clinical Science, School of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
    • ,
  • Hassan Momtaz, DVM, PhD

      Affiliations

    • Department of Microbiology and Biotechnology, Faculty of Veterinary Medicine, Azad University of Shahrekord, Shahrekord, Iran
    • ,
  • Ehsan Taheri, DVM

      Affiliations

    • Department of Clinical Science, Faculty of Veterinary Medicine, Azad University of Shahrekord, Shahrekord, Iran

References 

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  12. Deeg CA, Pompetzki D, Raith AJ, Hauck SM, Amann B, Suppmann S, et al. Identification and functional validation of novel autoantigens in equine uveitis. Mol Cell Proteomics. 2006;5:1462–1470
  13. Hartskeerl RA, Goris MG, Brem S, Meyer P, Kopp H, Gerhards H, et al. Classification of Leptospira from the eyes of horses suffering from recurrent uveitis. J Vet Med. 2004;51:110
  14. Robinson NE. Current therapy in equine medicine. 5th ed.. Philadelphia, PA: WB Saunders; 2003;
  15. Williams RD, Morter RL, Freeman MJ, Lavignette AM. Experimental chronic uveitis, ophtalmic signs following equine leptospirosis. Invest Ophthalmol. 1971;10:948–954
  16. Faber NA, Crawford M, LeFebvre FB, Buyukmihci NC, Madigan JE, et al. Detection of Leptospira spp. in the aqueous humor of horses with naturally acquired recurrent uveitis. J Clin Microbiol. 2000;38:2731–2733
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  24. Ahmad SN, Shah S, Ahmad FMH. Laboratory diagnosis of leptospirosis. J Postgrad Med. 2005;51:195–200
  25. Wollanke B, Rohrbach BW, Gerhards H. Serum and vitreous humor antibody titers in and isolation of Leptospira interrogans from horses with recurrent uveitis. J AmVet Med Assoc. 2001;219:795–800
  26. Hong CB, Donahue JM, Giles RC, Petrites-Murphy MB, Poonacha KB, Roberts AW, et al. Equine abortion and stillbirth in central Kentucky during 1988 and 1989 foaling seasons. J Vet Diagn Invest. 1993;5:560–566
  27. Ooteman MC, Vago AR, Koury MC. Evaluation of MAT, IgM ELISA and PCR methods for the diagnosis of human leptospirosis. J Microbiol Methods. 2005;65:247–257
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  29. Gilger BC, Malok E, Cutter KV, Stewart T, Horohov DW, Allen JB. Characterization of T-lymphocytes in the anterior uvea of eyes with chronic equine recurrent uveitis. Vet Immunol Immunopathol. 1999;71:17–28
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  31. Cetinkaya B, Basri Ertas H, Ongor H, Muz A. Detection of Leptospira species by polymerase chain reaction (PCR) in urine of cattle. Turk J Vet Anim Sci. 2000;24:123–130
  32. Gerritsen MJ, Olyhoek T, Smits MA, Bokhout BA. Sample preparation method for polymerase chain reaction-based semiquantitative detection of Leptospira interrogans serovar hardjo subtype hardjobovis in bovine urine. J Clin Microbiol. 1991;29:2805–2808
  33. Kim S, Lee DS, Suzuki H, Watarai M. Detection of Brucella canis and Leptospira interrogans in canine semen by multiplex nested PCR. J Vet Med Sci. 2006;68:615–618

PII: S0737-0806(09)00650-9

doi: 10.1016/j.jevs.2009.10.015

Journal of Equine Veterinary Science
Volume 29, Issue 11 , Pages 795-798 , November 2009

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