Journal of Equine Veterinary Science
Volume 29, Issue 9 , Pages 675-680 , September 2009

Effect of Centrifugation Technique on Post-storage Characteristics of Stallion Spermatozoa

  • Gary W. Webb, PhD, PAS

      Affiliations

    • Corresponding Author InformationReprint requests: Dr.Gary W. Webb, Missouri State University, Department of Agriculture, 901 South National, Springfield, MO 65897.
    • ,
  • M.M. Dean, MS

References 

  1. Jasko DJ, Moran M, Farlin ME, Squires EL. Effect of seminal plasma dilution or removal on spermatozoal motion characteristics of cooled stallion semen. Theriogenology. 1991;35:1059–1068
  2. Pruitt JA, Arns MJ, Pool KC. Seminal plasma influences recovery of equine sperm following in vitro culture (37°C) and cold-storage (5°C). Theriogenology. 1993;39:291
  3. Brinsko SP, Crockett EC, Squires EL. Effect of centrifugation and partial removal of seminal plasma on equine spermatozoal motility after cooling and storage. Theriogenology. 2000;54:129–136
  4. Jasko DJ, Martin JM, Squires EL. Effect of insemination volume and concentration of spermatozoa on embryo recovery in mares. Theriogenology. 1992;37:1233–1239
  5. Dawson GR, Webb GW, Pruitt JA, Loughin TM, Arns MJ. Effect of different processing techniques on motility and acrosomal integrity of cold-stored stallion spermatozoa. J Equine Vet Sci. 2000;20:191–194
  6. Love CC, Brinkso SP, Rigby SL, Thompson JA, Blanchard TL, Varner DD. Relationship of seminal plasma level and extender type to sperm motility and DNA integrity. Theriogenology. 2005;63:1584–1591
  7. Raphael CF, Andrade AFC, Nascimento J, Arruda RP. Effects of centrifugation on membrane integrity and lipid peroxidation of equine cooled spermatozoa. Anim Reprod Sci. 2008;107:344–345
  8. Dell'aqua JA, Papa FO, Alvarenga MA, Zahn FS. Effect of centrifugation and packing system on sperm parameters of equine frozen semen. Anim Reprod Sci. 2001;68:315
  9. Weiss S, Janett F, Burger D, Hassig M, Thun R. The influence of centrifugation on quality and freezability of stallion semen. Schweiz Arch Tierheilk. 2004;146:285–293
  10. Cochran JD, Amann RP, Froman DP, Pickett BW. Effects of centrifugation, glycerol level, cooling to 5oC, freezing rate and thawing rate on the post-thaw motility of equine sperm. Theriogenology. 1984;22:25–38
  11. Ecot P, Decuadro-Hansen G, Delhomme G, Vidament M. Evaluation of a cushioned centrifugation technique for processing equine semen for freezing. Anim Reprod Sci. 2005;89:245–248
  12. Sieme H, Knop K, Rath D. Effects of cushioned centrifugation on sperm quality in stallion semen stored cooled at 5 degrees C for 24 h, and stored cooled for 2 or 24 h and then frozen. Anim Reprod Sci. 2006;94:64–66
  13. Waite JA, Love CC, Brinsko SP, Teague SR, Salazar JL, Mancill SS, et al. Factors impacting equine sperm recovery rate and quality following cushioned centrifugation. Theriogenology. 2008;70:704–714

PII: S0737-0806(09)00574-7

doi: 10.1016/j.jevs.2009.07.016

Journal of Equine Veterinary Science
Volume 29, Issue 9 , Pages 675-680 , September 2009

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