Journal of Equine Veterinary Science
Volume 29, Issue 1 , Pages 24-32 , January 2009

Colloidal Centrifugation of Stallion Semen: Changes in Sperm Motility, Velocity, and Chromatin Integrity during Storage

  • J.M. Morrell

      Affiliations

    • Department of Clinical Sciences, Division of Reproduction, Swedish University of Agricultural Sciences, Uppsala
    • Corresponding Author InformationReprint requests: J. Morrell, Swedish University of Agricultural Sciences, SLU Department of Clinical Sciences, Division of Reproduction, Box 7054, Uppsala SE-75007.
  • ,
  • A. Johannisson

      Affiliations

    • Department of Anatomy, Physiology & Biochemistry, Swedish University of Agricultural Sciences, Uppsala, Sweden
  • ,
  • H. Strutz

      Affiliations

    • Department of Clinical Sciences, Division of Reproduction, Swedish University of Agricultural Sciences, Uppsala
  • ,
  • A.-M. Dalin

      Affiliations

    • Department of Clinical Sciences, Division of Reproduction, Swedish University of Agricultural Sciences, Uppsala
  • ,
  • H. Rodriguez-Martinez

      Affiliations

    • Department of Clinical Sciences, Division of Reproduction, Swedish University of Agricultural Sciences, Uppsala

References 

  1. Watson PF. Artificial insemination and the preservation of semen. In:  Lamming GE editors. Marshall´s physiology of reproduction. vol. 2:London: Churchill Livingstone; 1990;p. 772
  2. Colenbrander B, Gadella BM, Stout TAE. The predictive value of semen analysis in the evaluation of stallion fertility. Reprod Dom Anim. 2003;38:305–311
  3. Love CC. The sperm chromatin structure assay: a review of clinical applications. Anim Reprod Sci. 2005;89:39–25
  4. Morrell JM, Johannisson A, Dalin A-M, Hammar L, Sandebert T, Rodriguez-Martinez H. Sperm morphology and chromatin integrity in Swedish warmblood stallions and their relationship to pregnancy rates. Acta Vet Scand. 2008;50:2
  5. Malmgren L. Effectiveness of two systems for transporting equine semen. Theriogenology. 1998;50:833–839
  6. Tejerina F, Buranaamnuay K, Saravia F, Wallgren M, Rodriguez-Martinez H. Assessment of motility of ejaculated, liquid-stored boar spermatozoa using computerized instruments. Theriogenology. 2008;69:1129–1138
  7. Kenney RM, Bergman RV, Cooper WL, Morse GW. Minimal contamination techniques for breeding mares: techniques and preliminary findings. Proceedings American Association of Equine Practitioners. 1975;21:327–336
  8. Morrell JM, Dalin A-M, Rodriguez-Martinez H. Comparison of density gradient and single layer centrifugation of stallion spermatozoa: yield, motility and survival. Equine Vet J In press.
  9. Evenson DP, Darzynkiewicz Z, Melamed MR. Relation of mammalian sperm chromatin heterogeneity to fertility. Science. 1980;210:1131–1133
  10. Amann RP. Weaknesses in reports of “fertility” for horses and other species. Theriogenology. 2005;63:698–715
  11. Love CC, Kenney RM. The relationship of increased susceptibility of sperm DNA to denaturation and fertility in the stallion. Theriogenology. 1998;50:955–972
  12. Batellier F, Duchamp G, Vidament M, Arnaud G, Palmer E, Magistrini M. Delayed insemination is successful with a new extender for storing fresh equine semen at 15°C under aerobic conditions. Theriogenology. 1998;50:229–236
  13. Batellier F, Vidament M, Fauquant J, Duchamp G, Arnaud G, Yvon JM, et al. Advances in cooled semen technology. Anim Reprod Sci. 2001;68:181–190
  14. Olds-Clarke P. How does poor motility alter sperm fertilizing ability. J Androl. 1996;17:183–186
  15. Robayo I, Montenegro V, Valdes C, Cox JF. CASA assessment of kinematic parameters of ram spermatozoa and their relationship to migration efficiency in ruminant cervical mucus. Reprod Dom Anim. 2008;e pub
  16. Gillan L, Kroetsch T, Maxwell WM, Evans G. Assessment of in vitro sperm characteristics in relation to fertility in dairy bulls. Anim Reprod Sci. 2008;103:201–214
  17. Kirk ES, Squires EL, Graham JK. Comparison of in vitro laboratory analyses with the fertility of cryopreserved stallion spermatozoa. Theriogenology. 2005;64:1422–1439

 Refereed

PII: S0737-0806(08)00377-8

doi: 10.1016/j.jevs.2008.11.006

Journal of Equine Veterinary Science
Volume 29, Issue 1 , Pages 24-32 , January 2009