Journal of Equine Veterinary Science
Volume 28, Issue 11 , Pages 677-685 , November 2008

Semen Processing for the Subfertile Stallion

  • Dickson D. Varner, DVM, MS, Diplomate ACT

      Affiliations

    • Department of Large Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX
    • Corresponding Author InformationReprint requests: Dickson D. Varner, DVM, MS, Department of Large Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77843-4475.
    • ,
  • Charles C. Love, DVM, PhD, Diplomate ACT

      Affiliations

    • Department of Large Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX
    • ,
  • Steven P. Brinsko, DVM, PhD, Diplomate ACT

      Affiliations

    • Department of Large Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX
    • ,
  • Terry L. Blanchard, DVM, MS, Diplomate ACT

      Affiliations

    • Department of Large Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX
    • ,
  • David L. Hartman, DVM

      Affiliations

    • Hartman Equine Reproduction Center, P.A., Whitesboro, TX
    • ,
  • Shalyn B. Bliss, DVM

      Affiliations

    • Hartman Equine Reproduction Center, P.A., Whitesboro, TX
    • ,
  • Brian S. Carroll, DVM

      Affiliations

    • Oklahoma City Equine Clinic, Oklahoma City, OK
    • ,
  • Mark C. Eslick, DVM

      Affiliations

    • Hagyard Equine Medical Institute, Lexington, KY

References 

  1. Gebauer MR, Pickett BW, Voss JL, Swierstra EE. Reproductive physiology of the stallion: daily sperm output and testicular measurements. J Am Vet Med Assoc. 1974;165:711–713
  2. Thompson JA, Love DD, Stich KL, Brinsko SP, Blanchard TL, Varner DD. A Bayesian approach to prediction of stallion daily sperm output. Theriogenology;62:1607–1617.
  3. Love CC, Garcia MC, Riera FR, Kenney RM. Use of testicular volume to predict daily sperm output in the stallion. Proc Ann Conv Am Assoc Equine Pract. 1990;36:15–21
  4. Varner DD, Vaughan SD, Johnson L. Use of a computerized system for evaluation of equine spermatozoal motility. Am J Vet Res. 1991;52:224–230
  5. Evenson DP. Flow cytometry of acridine orange stained sperm is a rapid and practical method for monitoring occupational exposure to genotoxicants. In:  Sorsa M,  Norpa H editor. Monitoring of occupational genotoxicants. New York: Alan R. Liss; 1986;p. 121–132
  6. Love CC, Kenney RM. The relationship of increased susceptibility of sperm DNA to denaturation and fertility in the stallion. Theriogenology. 1998;52:955–972
  7. Morris LH, Hunter RH, Allen WR. Hysteroscopic insemination of small numbers of spermatozoa at the uterotubal junction of preovulatory mares. J Reprod Fertil. 2000;118:95–100
  8. Morris LH, Allen WR. An overview of low dose insemination in the mare. Reprod Dom Anim. 2002;37:206–210
  9. Morris LH. Low dose insemination in the mare: an update. Anim Reprod Sci. 2004;82-83:625–632
  10. Brinsko SP, Rigby SL, Lindsey AC, Blanchard TL, Love CC, Varner DD. Pregnancy rates in mares following hysteroscopic or transrectally-guided insemination with low sperm numbers at the utero-tubal papilla. Theriogenology. 2003;59:1001–1009
  11. Waite JA, Love CC, Brinsko SP, Teague SR, Salazar JL, Mancill SS, et al. Factors impacting equine sperm recovery rate and quality following cushioned centrifugation. Theriogenology. 2008;70:704–714
  12. Macpherson ML, Blanchard TL, Love CC, Brinsko SP, Varner DD. Use of a silane-coated silica particle solution to enhance the quality of ejaculated semen in stallions. Theriogenology. 2002;58:317–320
  13. Barth AD, Oko RJ. Nuclear vacuoles. In:  Barth AD,  Oko RJ editor. Abnormal morphology of bovine spermatozoa. Ames, IA: Iowa State University Press; 1989;p. 145–151
  14. Hurtgen JP, Johnson LA. Fertility of stallions with abnormalities of the sperm acrosome. J Reprod Fertil (Suppl). 1982;32:15–20
  15. Varner DD, Blanchard TL, Brinsko SP, Love CC, Taylor TS, Johnson L. Techniques for evaluating selected reproductive disorders of stallions. Anim Reprod Sci. 2000;60-61:493–509
  16. Varner DD, Brinsko SP, Blanchard TL, Love CC, Macpherson ML, Heck RS, et al. Subfertility in stallions associated with spermatozoal acrosome dysfunction. Proc Ann Conv Am Assoc Equine Pract. 2001;47:227–228
  17. Padilla AW, Foote RH. Extender and centrifugation effects on the motility patterns of slow-cooled stallion spermatozoa. J Anim Sci. 1991;69:3308–3313
  18. Rigby SL, Brinsko SP, Cochran M, Blanchard TL, Love CC, Varner DD. Advances in cooled semen technologies: seminal plasma and semen extender. Anim Reprod Sci. 2001;68:171–180
  19. Blanchard TL, Varner DD, Schumacher J, Love CC, Brinsko SP, Rigby SL. Semen collection and artificial insemination. In:  Blanchard TL,  Varner DD,  Schumacher J,  Love CC,  Brinsko SP,  Rigby SL editor. Manual of equine reproduction. 2nd ed.. St. Louis: Mosby; 2003;p. 131–142
  20. Blanchard TL, Love CC, Thompson JA, Ramsey J, O'Meara A. Role of reinforcement breeding in a natural service mating program. Proc Ann Conv Am Assoc Equine Pract. 2006;52:384–386

 Refereed

PII: S0737-0806(08)00342-0

doi: 10.1016/j.jevs.2008.10.012

Journal of Equine Veterinary Science
Volume 28, Issue 11 , Pages 677-685 , November 2008

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