Journal of Equine Veterinary Science
Volume 28, Issue 11 , Pages 672-676 , November 2008

Cryopreservation of Stallion Spermatozoa with INRA96 and Glycerol

  • Richard Fayrer-Hosken

      Affiliations

    • Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens, GA
    • Primary author.
    • Corresponding Author InformationReprint requests: Richard Fayrer-Hosken, BVSc, PhD, Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens, GA 30602-7385.
    • ,
  • Christine Abreu-Barbosa

      Affiliations

    • Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens, GA
    • ,
  • Gary Heusner

      Affiliations

    • Department of Animal Sciences, College of Agriculture, University of Georgia, Athens, GA
    • ,
  • Lorelei Jones

      Affiliations

    • Department of Animal Sciences, College of Agriculture, University of Georgia, Athens, GA

References 

  1. Woods EJ, Benson JD, Agca Y, Critser JK. Fundamental cryobiology of reproductive cells and tissues. Cryobiology. 2004;48:146–156
  2. Alvarenga MA, Papa FO, Landim-Alvarenga FC, Medeiros AS. Amides as cryoprotectants for freezing stallion semen: a review. Anim Reprod Sci. 2005;89:105–113
  3. Polge C, Smith AU, Parkes AS. Revival of spermatozoa after vitrification and dehydration at low temperatures. Nature. 1949;164:666–676
  4. Guay P, Rondeau M, Boucher S. Effect of glycerol on motility, viability, extracellular aspartate aminotransferase release and fertility of stallion semen before and after freezing. Equine Vet J. 1981;13:177–182
  5. Alvarenga MA, Landim-Alvarenga FC, Moreira RM, Cesarino MM. Acrosomal ultrastructure of stallion spermatozoa cryopreserved with ethylene glycol using two packaging systems. Equine Vet J. 2000;32:541–545
  6. Squires EL, Keith SL, Graham JK. Evaluation of alternative cryoprotectants for preserving stallion spermatozoa. Theriogenology. 2004;62:1056–1065
  7. Cochran JD, Amann RP, Froman DP, Pickett BW. Effects of centrifugation, glycerol level, cooling to 5°C, freezing rate and thawing rate on the post-thaw motility of equine sperm. Theriogenology. 1984;22:25–38
  8. Cristanelli MJ, Amann RP, Squires EL, Pickett BW. Effects of egg yolk and glycerol levels in lactose-EDTA-egg yolk extender and of freezing rate on the motility of frozen-thawed stallion spermatozoa. Theriogenology. 1985;24:681–686
  9. DeJarnette JM, Barnes DA, Marshall CE. Effects of pre- and post-thaw thermal insults on viability characteristics of cryopreserved bovine semen. Theriogenology. 2000;53:1225–1238
  10. Loomis PR, Amann RP, Squires EL, Pickett BW. Fertility of unfrozen and frozen stallion spermatozoa extended in EDTA-lactose-egg yolk and packaged in straws. J Anim Sci. 1983;56:687–693
  11. Kenney RM, Bergman RV, Cooper WL, Morse GW. Minimal contamination techniques for breeding mares: techniques and preliminary findings. Proc Am Assoc Equine Pract. 1975;327–336
  12. Garcia MA, Graham EF. Dialysis of bovine semen and its effect on fresh and freeze-thawed spermatozoa. Cryobiology. 1987;24:446–454
  13. Batellier F, Magistrini M, Fauquant J, Palmer E. Effect of milk fractions on survival of equine spermatozoa. Theriogenology. 1996;48:391–410
  14. Leboeuf B, Guillouet P, Batellier F, Bernelas D, Bonne JL, Forgerit Y, et al. Effect of native phosphocaseinate on the in vitro preservation of fresh semen. Theriogenology. 2003;60:867–877
  15. Ellington JE, Ball BA, Blue BJ, Wilker CE. Capacitation-like membrane changes and prolonged viability in vitro of equine spermatozoa cultured with uterine tube epithelial cells. Am J Vet Res. 1993;54:1505–1510
  16. Thomas AD, Meyers SA, Ball BA. Capacitation-like changed in equine spermatozoa following cryopreservation. Theriogenology. 2006;65:1531–1550
  17. Vidament M, Dupere AM, Julienne P, Evain A, Noue P, Palmer E. Equine frozen semen: Freezability and fertility field results. Theriogenology. 1997;48:905–917
  18. Magistrini M, Chanteloube P, Palmer E. Influence of season and frequency of ejaculation on production of stallion semen for freezing. J Reprod Fertil (Suppl). 1987;35:127–133
  19. Crockett EC, Graham JK, Bruemmer JE, Squires EL. Effect of cooling of equine spermatozoa before freezing on post-thaw motility: preliminary results. Theriogenology. 2001;55:793–803
  20. Bedford SJ, Graham JK, Amann RP, Squires EL, Pickett BW. Use of two freezing extenders to cool stallion spermatozoa to 5 degrees C with and without seminal plasma. Theriogenology. 1995;43:939–953
  21. Loomis PR. Advanced methods for handling and preparation of stallion semen. Vet Clin North Am: Equine Pract. 2006;22:663–676
  22. Moore AI, Squires EL, Graham JK. Effect of seminal plasma on the cryopreservation of equine spermatozoa. Theriogenology. 2005;63:2372–2381
  23. Samper J, Jensen S, Sergeant J, Estrada A. Timing of induction of ovulation in mares treated with Ovuplant or Chorulon. J Equine Vet Sci. 2002;22:320–323
  24. In:  Samper J editors. Equine breeding management and artificial insemination. vol. 1:Philadelphia: W.B. Saunders Company; 2000;36
  25. White IG. Lipids and calcium uptake of sperm in relation to cold shock and preservation: a review. Reprod Fertil Dev. 1993;5:639–658

 Refereed

PII: S0737-0806(08)00336-5

doi: 10.1016/j.jevs.2008.10.006

Journal of Equine Veterinary Science
Volume 28, Issue 11 , Pages 672-676 , November 2008

Article Tools