Journal of Equine Veterinary Science
Volume 28, Issue 11 , Pages 650-665 , November 2008

Diagnostic Methods for Evaluation of Stallion Subfertility: A Review

  • Barry A. Ball, DVM, PhD, Diplomate A.C.T.

      Affiliations

    • Corresponding Author InformationReprint requests: Barry A. Ball, DVM, PhD, Room 1133, Tupper Hall, University of California, Davis, Davis, CA 95616.

References 

  1. Gebauer MR, Pickett BW, Voss JL, Swierstra EE. Reproductive physiology of the stallion: daily sperm output and testicular measurements. J Am Vet Med Assoc. 1974;165:711–713
  2. Love CC, Garcia MC, Riera FR, Kenney RM. Evaluation of measures taken by ultrasonography and calipers to estimate testicular volume and predict daily sperm output in the stallion. J Reprod Fertil Suppl. 1991;44:99–105
  3. Love CC. Ultrasonographic evaluation of the testis, epididymis, and spermatic cord of the stallion. Vet Clin North Am Equine Pract. 1992;8:167–182
  4. Pozor M. Diagnostic applications of ultrasonography to stallion's reproductive tract. Theriogenology. 2005;64:505–509
  5. Stallion Ginther OJ. Ultrasonic imaging and animal reproduction: horses, book 2. Cross Plains, WI: Equiservices Publishing. 1995;309–336
  6. Little TV, Woods GL. Ultrasonography of the accessory sex glands in the stallion. J Reprod Fertil Suppl. 1987;35:87–94
  7. Pozor MA, McDonnell SM. Color Doppler ultrasound evaluation of testicular blood flow in stallions. Theriogenology. 2004;61:799–810
  8. Ginther OJ. Blood flow of male genitalia. In: Ultrasonic imaging and animal reproduction: Doppler. Cross Plains, WI: Equiservices Publishing. 2007;205–224
  9. Pozor MA. Evaluation of testicular vasculature in stallions. Clin Tech Equine Pract. 2007;6:271–277
  10. McEntee KE. Reproductive pathology of domestic animals. San Diego, CA: Academic Press; 1990;
  11. Edwards JF. Pathologic conditions of the stallion reproductive tract. Anim Reprod Sci. 2008;107:197–207
  12. Brown JA, O'Brien MA, Hodder ADJ, Peterson T, Claes A, Liu IKM, et al. Unilateral testicular mastocytoma in a Peruvian Paso stallion. Equine Vet Educ. 2008;20:172–175
  13. Beck C, Charles JA, MacLean AA. Ultrasound appearance of an equine testicular seminoma. Vet Radiol Ultrasound. 2001;42:355–357
  14. Varner DD, Schumacher J, Blanchard TL, Johnson L. Diseases and management of breeding stallions. Goleta, CA: American Veterinary Publications; 1991;
  15. Morresey PR. The enlarged scrotum. Clin Tech Equine Pract. 2007;6:265–270
  16. Hyland J, Church S. The use of ultrasonograph in the diagnosis and treatment of a haematoma in the corpus cavernosum penis of a stallion. Aust Vet J. 1995;72:468–469
  17. Weber JA, Geary RT, Woods GL. Changes in accessory sex glands of stallions after sexual preparation and ejaculation. J Am Vet Med Assoc. 1990;196:1084–1089
  18. Varner DD, Blanchard TL, Brinsko SP, Love CC, Taylor TS, Johnson L. Techniques for evaluating selected reproductive disorders of stallions. Anim Reprod Sci. 2000;60–61:493–509
  19. Estrada A, Samper JC, Lillich JD, Rathi R, Brault LS, Albrecht BA, et al. Azoospermia associated with bilateral segmental aplasia of the ductus deferens in a stallion. J Am Vet Med Assoc. 2003;222:1740–1742
  20. Jann HW, Rains JR. Diagnostic ultrasonography for evaluation of cryptorchidism in horses. J Am Vet Med Assoc. 1990;196:297–300
  21. Schambourg MA, Farley JA, Marcoux M, Laverty S. Use of transabdominal ultrasonography to determine the location of cryptorchid testes in the horse. Equine Vet J. 2006;38:242–245
  22. Sullins KE, Bertone JJ, Voss JL, Pederson SJ. Treatment of hemospermia in stallions: a discussion of 18 cases. Comp Contin Educ Pract Vet. 1988;10:1395–1403
  23. Faber NF, Roser JF. Testicular biopsy in stallions: diagnostic potential and effects on prospective fertility. J Reprod Fertil Suppl. 2000;56:31–42
  24. Bartmann CP, Schoon HA, Lorber K, Brickwedel I, Klug E. Testicular sonography and biopsy in the stallion—indication, techniques and diagnostic relevance. Pferdeheilkunde. 1999;15:506
  25. DelVento VR, Amann RP, Trotter GW, Veeramachaneni DNR, Squires EL. Ultrasonographic and quantitative histologic assessment of sequelae to testicular biopsy in stallions. Am J Vet Res. 1992;53:2094–2101
  26. Leme DP, Papa FO. Cytological identification and quantification of testicular cell types using fine needle aspiration in horses. Equine Vet J. 2000;32:444–446
  27. Turner RMO, McDonnell SM. Alkaline phosphatase in stallion semen: characterization and clinical applications. Theriogenology. 2003;60:1–10
  28. Kenney RM, Hurtgen JP, Pierson R, Witherspoon DM, Simons J. Clinical fertility evaluation of the stallion. Proc Soc Theriogenol. 1983;9:1–100
  29. Griggers S, Paccamonti DL, Thompson RA, Eilts BE. The effects of pH, osmolarity and urine contamination on equine spermatozoal motility. Theriogenology. 2001;56:613–622
  30. Devireddy RV, Swanlund DJ, Alghamdi AS, Duoos LA, Troedsson MHT, Bischof JC, et al. Measured effect of collection and cooling conditions on the motility and the water transport parameters at subzero temperatures of equine spermatozoa. Reproduction. 2002;124:643–648
  31. Althouse GC, Seager SWJ, Varner DD, Webb GW. Diagnostic aids for the detection of urine in the equine ejaculate. Theriogenology. 1989;31:1141–1148
  32. Dascanio JJ, Witonsky SG. Theriogenology question of the month. J Am Vet Med Assoc. 2005;227:225–227
  33. Dowsett KF, Pattie WA. Characteristics and fertility of stallion semen. J Reprod Fertil Suppl. 1982;32:1–8
  34. Jasko DJ, Lein DH, Foote RH. Determination of the relationship between sperm morphologic classifications and fertility in stallions: 66 cases (1987–1988). J Am Vet Med Assoc. 1990;197:389–394
  35. Kenney RM, Evenson DP, Garcia MC, Love C. Relationship between sperm chromatin structure, motility, and morphology of ejaculated sperm and seasonal pregnancy. In:  Sharp DC,  Bazer FW editor. Biology of reproduction monograph series 1, equine reproduction VI. Ann Arbor, MI: Edwards Brothers, Inc.; 1995;p. 647–654
  36. Hurtgen JP, Johnson LA. Fertility of stallions with abnormalities of the sperm acrosome. J Reprod Fertil Suppl. 1982;32:15–20
  37. Johansson CS, Matsson FC, Lehn-Jensen H, Nielsen JM, Petersen MM. Equine spermatozoa viability comparing the NucleoCounter SP-100 and the eosin-nigrosin stain. Anim Reprod Sci. 2008;107:325–326
  38. Rousset H, Chanteloube P, Magistrini M, Palmer E. Assessment of fertility and semen evaluations of stallions. J Reprod Fertil Suppl. 1986;35:25–31
  39. Veeramachaneni DNR, Moeller CL, Sawyer HR. Sperm morphology in stallions: ultrastructure as a functional and diagnostic tool. Vet Clin North Am Equine Pract. 2006;22:683–692
  40. Varner DD. Developments in stallion semen evaluation. Theriogenology. 2008;70:448–462
  41. Gravance CG, Garner DL, Baumber J, Ball BA. Assessment of equine sperm mitochondrial function using JC-1. Theriogenology. 2000;53:1691–1703
  42. Ball BA, Vo A. Detection of lipid peroxidation in equine spermatozoa based upon the lipophilic fluorescent dye C-11-BODIPY581/591. J Androl. 2002;23:259–269
  43. Ball BA. Oxidative stress, osmotic stress and apoptosis: impacts on sperm function and preservation in the horse. Anim Reprod Sci. 2008;107:257–267
  44. Burnaugh L, Sabeur K, Ball BA. Generation of superoxide anion by equine spermatozoa as detected by dihydroethidium. Theriogenology. 2007;67:580–589
  45. Sabeur K, Ball BA. Detection of superoxide anion generation by equine spermatozoa. Am J Vet Res. 2006;67:701–706
  46. Ball BA, Vo AT, Baumber J. Generation of reactive oxygen species by equine spermatozoa. Am J Vet Res. 2001;62:508–515
  47. Thomas AD, Meyers SA, Ball BA. Capacitation-like changes in equine spermatozoa following cryopreservation. Theriogenology. 2006;65:1531–1550
  48. Brum AM, Sabeur K, Ball BA. Apoptotic-like changes in equine spermatozoa separated by density-gradient centrifugation or after cryopreservation. Theriogenology. 2008;69:1041–1055
  49. Bosard T, Love C, Brinsko S, Blanchard T, Thompson J, Varner D. Evaluation and diagnosis of acrosome function/dysfunction in the stallion. Anim Reprod Sci. 2005;89:215–217
  50. Brum AM, Thomas AD, Sabeur K, Ball BA. Evaluation of Coomassie blue staining of the acrosome of equine and canine spermatozoa. Am J Vet Res. 2006;67:358–362
  51. Thomas PGA, Ball BA, Miller PG, Brinsko SP, Southwood L. A subpopulation of morphologically normal, motile spermatozoa attach to equine oviduct epithelial cells in vitro. Biol Reprod. 1994;51:303–309
  52. Dobrinski I, Thomas PGA, Ball BA. Cryopreservation reduces the ability of equine spermatozoa to attach to oviductal epithelial cells and zonae pellucidae in vitro. J Androl. 1995;16:536–542
  53. Meyers SA, Liu IKM, Overstreet JW, Vadas S, Drobnis EZ. Zona pellucida binding and zona-induced acrosome reactions in horse spermatozoa: comparisons between fertile and subfertile stallions. Theriogenology. 1996;46:1277–1288
  54. Evenson DP, Larson KL, Jost LK. Sperm chromatin structure assay: its clinical use for detecting sperm DNA fragmentation in male infertility and comparisons with other techniques. J Androl. 2002;23:25–43
  55. Love CC, Kenney RM. The relationship of increased susceptibility of sperm DNA to denaturation and fertility in the stallion. Theriogenology. 1998;50:955–972
  56. Love CC, Kenney RM. Scrotal heat stress induces altered sperm chromatin structure associated with a decrease in protamine disulfide bonding in the stallion. Biol Reprod. 1999;60:615–620
  57. Baumber J, Ball BA, Linfor JJ, Meyers SA. Reactive oxygen species and cryopreservation promote deoxyribonucleic acid (DNA) damage in equine sperm. Theriogenology. 2002;58:301–302
  58. Roser JF. Endocrine diagnostics for stallion infertility. In: Ball BA, ed. Recent advances in equine reproduction. Ithaca, NY: International Veterinary Information Service; 2001. Available from: URL: http://www.ivis.org/advances/Reproduction_Ball/stallion_fertility_roser/ivis.pdf.
  59. Roser JF. Regulation of testicular function in the stallion: An intricate network of endocrine, paracrine and autocrine systems. Anim Reprod Sci. 2008;107:179–196
  60. Kenney RM, Kent MG, Garcia MC, Hurtgen JP. The use of DNA index and karyotype analyses as adjuncts to the estimation of fertility in stallions. J Reprod Fertil Suppl. 1991;44:69–75
  61. Leeb T. The horse genome project—sequence based insights into male reproductive mechanisms. Reprod Dom Anim. 2007;42:45–50
  62. Hamann H, Jude R, Sieme H, Mertens U, Topfer-Petersen E, Distl O, et al. A polymorphism within the equine CRISP3 gene is associated with stallion fertility in Hanoverian warmblood horses. Anim Genet. 2007;38:259–264

 Refereed

PII: S0737-0806(08)00333-X

doi: 10.1016/j.jevs.2008.10.003

Journal of Equine Veterinary Science
Volume 28, Issue 11 , Pages 650-665 , November 2008

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