Journal of Equine Veterinary Science
Volume 28, Issue 6 , Pages 351-358 , June 2008

Effects of Sperm Concentration and Straw Volume on Motion Characteristics and Plasma, Acrosomal, and Mitochondrial Membranes of Equine Cryopreserved Spermatozoa

References 

  1. Neves Neto JR, Papa FO, Leme DP. Avaliação entre congelabilidade e fertilidade do sêmen eqüino frente a dois diluidores. Rev Bras Reprod Anim. 1989;23:301–302
  2. Marshburn PB, McIntire D, Carr BR, Byrd W. Spermatozoal characteristics from fresh and frozen donor semen and their correlation with fertility outcome after intrauterine insemination. Fertil Steril. 1992;58:179–186
  3. Samper J, Morris CA. Current methods for stallion semen cryopreservation: a survey. Theriogenology. 1998;49:895–903
  4. Watson PF. The causes of reduced fertility with cryopreserved semen. Anim Reprod Sci. 2000;60-61:481–492
  5. Blach EL, Amann RP, Bowen RA, Frantz D. Changes in quality of stallion spermatozoa during cryopreservation plasma membrane integrity and motion characteristics. Theriogenology. 1989;31:283–298
  6. Merkt H, Klug E, Krause D, Bader H. Results of long-term storage of stallion semen frozen by the pellet method. J Reprod Fertil. 1975;23(Suppl):105–106
  7. Suh TK, Schenk JL, Seidel GE. High pressure flow cytometric sorting damages sperm. Theriogenology. 2005;64:1035–1048
  8. Love CC, Loch WL, Bristol F, Garcia MC, Kenney RM. Comparison of pregnancy rates achieved with frozen semen using two packaging methods. Theriogenology. 1989;31:613–622
  9. Arruda RP, Vieira RC, Valente Filho JB, Visintin JÁ, Neves Neto JR. Utilização de uma seringa modificada para aplicação do sêmen eqüino congelado em bainhas plásticas e macrotubos. ARS Vet. 1994;10:242
  10. Loomis PR, Amann RP, Squires EL, Pickett BW. Fertility of unfrozen stallion spermatozoa extended in EDTA-lactose-egg yolk and packed in straws. J Anim Sci. 1983;56:687–693
  11. Martin JC, Klug E, Guntzel AR. Centrifugation of stallion semen and its storage in large volume straws. J Reprod Fertil. 1979;27(Suppl):47–51
  12. Papa FO, Prestes NC, Alvarenga MA, Bicudo SD, Branco MDL. Influência de diferentes volumes sobre os parâmetros espermáticos e sobre os índices de fertilidade de sêmen congelado de eqüino. Rev Bras Reprod Anim. 1989;1(Suppl):223
  13. Loomis RP, Clark JS. Motion characteristics of frozen-thawed equine spermatozoa packed in 0.5 mL straws at various concentration. Proceedings Society for Theriogenology; 1998:142.
  14. Cristanelli MJ, Squires EL, Amann RP, Pickett BW. Fertility of stallion semen processed, frozen and thawed by a new procedure. Theriogenology. 1984;22:39–45
  15. Heitland AV, Jasko DJ, Squires EL, Graham JK, Pickett BW, Hamilton C. Factors affecting motion characteristics of frozen-thawed stallion spermatozoa. Equine Vet J. 1996;28:47–53
  16. Jasko DJ, Moran DM, Farlin ME, Squires EL, Amann RP, Pickett BW. Pregnancy rates utilizing fresh, cooled and frozen-thawed stallion semen. Proceedings 38thAnnual Convention AAEP; 1992:649–660.
  17. Squires EL, Pickett BW, Graham JK, Vanderwall DK, McCue PM, Bruemmer JE. Factors affecting fertility of frozen-thawed spermatozoa: cooled and frozen stallion semen. Fort Collins, CO: Colorado State University Animal Reproduction and Biotechnology Laboratory Bulletin No. 09; 1999;61–77
  18. Volkmann DH, Van-Zyl D. Fertility of stallion semen frozen in 0.50 mL straws. J Reprod Fertil. 1987;35:143–148
  19. Graham JK. Cryopreservation of stallion spermatozoa. Vet Clin North Am Equine Pract. 1996;12:131–147
  20. Hammerstedt RH, Drichton EG, Watson PF. Comparative approach to sperm cryopreservation: doses cell shape and size influence cryo-survival? Proceedings Society for Theriogenology; 1991:8–11.
  21. Angola AP, Mendez JV, Quintero LZ. Efecto del sistema de envasado y la concentración espermática sobre el daño acrosomal y la motilidad posdescongelación del semen equino. Vet Méx XXIII. 1992;44:315–318
  22. Buchanan BR, Seidel GE, McCue PM, Schenk JL, Herickhoff LA, Squires EL. Insemination of mares with low numbers of either unsexed or sexed spermatozoa. Theriogenology. 2000;53:1333–1344
  23. Lindsey AC, Bruemmer JE, Squires EL. Low dose insemination of mares using non-sorted and sex-sorted sperm. Anim Reprod Sci. 2001;68:279–289
  24. Petersen MM, Wessel MT, Scott MA, Liu IKM, Ball BA. Embryo recovery rates in mares after deep intrauterine insemination with low numbers of cryopreserved equine spermatozoa. Theriogenology. 2002;58:663–665
  25. Brinsko SP, Rigby SL, Lindsey AC, Blanchard TL, Love CC, Varner DD. Pregnancy rates in mares following hysteroscopic or transrectally-guided insemination with low sperm numbers at the utero-tubal papilla. Theriogenology. 2003;59:1001–1009
  26. Manning ST, Bowman PA, Fraser LM, Card CE. Development of hysteroscopic insemination of the uterine tube in the mare. Proceedings Annual Meeting Society for Theriogenology; 1998:84–85.
  27. Vazquez JJ, Medina V, Liu IK, Ball BA, Scott MA. Nonsurgical utero-tubal insemination in the mare. Proceedings Annual Meeting Society for Theriogenology; 1998:82–83.
  28. Lindsey AC, Varner DD, Seidel GE, Bruemmer JE, Squires EL. Hysteroscopic or rectally guided deep-uterine insemination of mares with spermatozoa stored 18 h at either 5 degrees C or 15 degrees C prior to flow-cytometric sorting. Anim Reprod Sci. 2005;85:125–130
  29. Verstegen J, Iguer-Ouada M, Onclin K. Computer assisted semen analyzer in Andrology research and veterinary practice. Theriogenology. 2002;57:149–179
  30. Kavak A, Johannisson N, Lundehein N, Rodriguez-Martinez H, Aidnik M, Einarsson S. Evaluation of cryopreserved stallion semen from Tori and Estonian breeds using CASA and flow cytometry. Anim Reprod Sci. 2003;76:205–216
  31. Rodriguez-Martinez H. Laboratory semen assessment and prediction of fertility: still utopia?. Reprod Dom Anim. 2003;28:5–6
  32. Kenney RM, Bergman RV, Cooper WL, Morse GW. Minimal contamination techniques for breeding mares and preliminary findings. Proceedings American Association Equine Practictioners; 1975:327–336.
  33. Bavister BD, Leibfried ML, Lieberman G. Development of preimplantation embryos of the golden hamster in a defined culture medium. Biol Reprod. 1983;28:235–247
  34. Celeghini ECC, Arruda RP, Andrade AFC, Raphael CF, Nascimento J. Simultaneous evaluation of the plasma, acrosomal and mitochondrial membranes in equine spermatozoa. Proceedings International Congress Animal Reproduction, 2004;15:511.
  35. Crockett ET, Graham JK, Bruemmer JE, Squires EL. Effect of cooling of equine spermatozoa before freezing on post-thaw motility; preliminary results. Theriogenology. 2001;55:793–803
  36. Blanes MS, Papa FO, Dores CB, Melo CM, Crocci AJ. Influência do número de espermatozóides e tempo de estabilização na congelação de sêmen eqüino utilizando-se diluente Botu-Crio. Acta Sci Vet. 2005;33:303
  37. Shivaji S, Peedicayil J, Devi GL. Analyses of the motility parameters of in vitro hyperactivated hamster spermatozoa. Mol Reprod Dev. 1995;42:233–247
  38. Jayaprakash D, Kumar SK, Shivaji S, Seshagiri PB. Pentoxifylline induces hyperactivation and acrosome reaction in spermatozoa of golden hamsters: changes in motility kinematics. Hum Reprod. 1997;12:2192–2199
  39. Grondall C, Grondall ML, Hyttel P, Greve T. Acrosomal status in fresh and frozen/thawed stallion spermatozoa evaluated by scanning electron microscopy. Anat Embryol. 1994;190:195–200
  40. Amann RP, Pickett BW. Principles of cryopreservation and a review of cryopreservation of stallion spermatozoa. J Equine Vet Sci. 1987;7:145–173
  41. Belous AM, Luguvoj VI, Moiseyev VA, Nardid OA, Zagnoyko VI. Examination by fluorescence and EPR spectroscopy of the state of mitochondrial and lysosomal membranes upon freeze–thawing. Cryobiology. 1990;27:184–188
  42. Neild DM, Gadella BM, Chaves MG, Miragaya MH, Colenbrander B, Aguero A. Membrane changes during different stages of a freeze–thaw protocol for equine semen cryopreservation. Theriogenology. 2003;59:1693–1705
  43. Blottner S, Warnke C, Tuchscherer A, Heinen V, Torner H. Morphological and functional changes of stallion spermatozoa after cryopreservation during breeding and non-breeding season. Anim Reprod Sci. 2001;65:75–88
  44. Schober D, Aurich C, Nohl H, Gille L. Influence of cryopreservation on mitochondrial functions in equine spermatozoa. Theriogenology. 2007;68:745–754
  45. Pena A, Linde-Forsberg C. Effects of spermatozoal concentration and post-thaw dilution rate on survival after thawing of dog spermatozoa. Theriogenology. 2000;54:703–718
  46. Celeghini ECC, Arruda RP, Andrade AFC, Nascimento J, Raphael CF, Rodriguez PHM. Effects that bovine sperm cryopreservation using two different extenders has on sperm membranes and chromatin. Anim Reprod Sci. 2008;104:119–131
  47. Nagy S, Jansen J, Topper EK, Gadella BM. A triple-stain flow cytometric method to assess plasma and acrosome membrane integrity of cryopreserved bovine sperm immediately after thawing in presence of egg-yolk particles. Biol Reprod. 2003;68:1828–1825
  48. Martinez-Pastor F, Johannisson A, Gil J, Kaabi M, Anel L, Paz P, et al. Use of chromatin stability assay, mitochondrial stain JC-1, and fluorometric assessment of plasma membrane to evaluate frozen-thawed ram semen. Anim Reprod Sci. 2004;84:121–133
  49. Mukai C, Okuno M. Glycolysis plays a major role for adenosine triphosphate supplementation in mouse sperm flagellar movement. Biol Reprod. 2004;71:540–547
  50. Silva PFN, Gadella BM. Detection of damage in mammalian sperm cells. Theriogenology. 2006;65:958–978
  51. Parks JE, Graham JK. Effects of cryopreservation procedures on sperm membranes. Theriogenology. 1992;38:209–222
  52. Darin-Bennett A, White IG. Influence of the cholesterol content of mammalian spermatozoa on susceptibility to cold-shock. Cryobiology. 1977;14:466–470
  53. Moore AI, Squires EL, Graham JK. Adding cholesterol to the stallion sperm plasma membrane improves cryosurvival. Cryobiology. 2005;51:241–249
  54. Squires EL, Pickett BW, Graham JK, Vanderwall DK, McCue PM, Bruemmer JE. Biology and structure of spermatozoa and their response to cooling. In: Cooled and frozen stallion semen. Fort Collins: Colorado State University Animal Reproduction and Biotechnology Laboratory Bulletin No. 09; 1999;3–9
  55. Gadella BM, Rathi R, Brouwers JFHM, Stout TAE, Colenbrander B. Capacitation and the acrosome reaction in equine sperm. Anim Reprod Sci. 2001;68:249–265
  56. Mazur P. Basic concepts in freezing cells. Proceedings First International Conference on Deep Freezing of Boar Semen; 1985:91–111.

 Refereed

PII: S0737-0806(08)00163-9

doi: 10.1016/j.jevs.2008.04.010

Journal of Equine Veterinary Science
Volume 28, Issue 6 , Pages 351-358 , June 2008