Journal of Equine Veterinary Science
Volume 28, Issue 3 , Pages 149-155 , March 2008

Evaluation of Plasmid Delivery by Electroporation as a Means of Increasing Gonadotropin-Releasing Hormone Production in Stallions

  • W.A. Storer

      Affiliations

    • School of Animal Sciences, Louisiana Agriculture Experiment Station, LSU Agricultural Center, Baton Rouge, LA
    • ,
  • D.L. Thompson Jr.

      Affiliations

    • School of Animal Sciences, Louisiana Agriculture Experiment Station, LSU Agricultural Center, Baton Rouge, LA
    • Corresponding Author InformationReprint requests: D.L. Thompson, Jr., School of Animal Sciences, Louisiana State University, Baton Rouge, LA 70803
    • ,
  • K.R. Bondioli

      Affiliations

    • School of Animal Sciences, Louisiana Agriculture Experiment Station, LSU Agricultural Center, Baton Rouge, LA
    • ,
  • R.A. Godke

      Affiliations

    • School of Animal Sciences, Louisiana Agriculture Experiment Station, LSU Agricultural Center, Baton Rouge, LA
    • ,
  • A.S. Khan

      Affiliations

    • VGX Pharmaceuticals, Immune Therapeutics Division, The Woodlands, TX
    • ,
  • P.A. Brown

      Affiliations

    • VGX Animal Health, The Woodlands, TX
    • ,
  • R. Draghia-Akli

      Affiliations

    • VGX Pharmaceuticals, Immune Therapeutics Division, The Woodlands, TX

References 

  1. Blue BJ, Pickett BW, Squires EL, McKinnon AO, Nett TM, Amann RP, et al. Effect of pulsatile or continuous administration of GnRH on reproductive function of stallions. J Reprod Fertil Suppl. 1991;44:145–154
  2. Boyle MS, Skidmore J, Zhang J, Cox JE. The effects of continuous treatment of stallions with high levels of a potent GnRH analogue. J Reprod Fertil Suppl. 1991;44:169–182
  3. Johnson CA, Thompson DL, Cartmill JA. Effects of deslorelin acetate implants in horses: single implants in stallions and steroid-treated geldings and multiple implants in mares. J Anim Sci. 2003;81:1300–1307
  4. Lucas ML, Heller R. Immunomodulation by electrically enhanced delivery of plasmid DNA encoding IL-12 to murine skeletal muscle. Mol Ther. 2001;3:47–53
  5. Lesbordes JC, Bordet T, Haase G, Castelnau-Ptakhine L, Rouhani S, Gilgenkrantz H, et al. In vivo electrotransfer of the cardiotrophin-1 gene into skeletal muscle slows down progression of motor neuron degeneration in pmn mice. Hum Mol Genet. 2002;11:1615–1625
  6. Terada Y, Tanaka H, Okado T, Inoshita S, Kuwahara M, Akiba T, et al. Efficient and ligand-dependent regulated erythropoietin production by naked DNA injection and in vivo electroporation. Am J Kidney Dis. 2001;38:S50–S53
  7. Yasui A, Oda K, Usunomiya H, Kakudo K, Suzuki T, Yoshida T, et al. Elevated gastrin secretion by in vivo gene electroporation in skeletal muscle. Int J Mol Med. 2001;8:489–494
  8. Fewell JG, MacLaughlin F, Mehta V, Gondo M, Nicol F, Wilson E, et al. Gene therapy for the treatment of hemophilia B using PINC-formulated plasmid delivered to muscle with electroporation. Mol Ther. 2001;3:574–583
  9. Draghia-Akli R, Cummings KK, Khan AS, Brown PA, Carpenter RH. Effects of plasmid-mediated growth hormone releasing hormone supplementation in young, healthy Beagle dogs. J Anim Sci. 2003;81:2301–2310
  10. Draghia-Akli R, Ellis KM, Hill LA, Malone PB, Fiorotto ML. High-efficiency growth hormone-releasing hormone plasmid vector administration into skeletal muscle mediated by electroporation in pigs. FASEB J. 2003;17:526–528
  11. Draghia-Akli R, Fiorotto ML. A new plasmid-mediated approach to supplement somatotropin production in pigs. J Anim Sci. 2004;82:E-Suppl:E264–E269
  12. Weesner GD, Becker BA, Matteri RL. Expression of luteinizing hormone-releasing hormone and its receptor in porcine immune tissues. Life Sci. 1997;61:1643–1649
  13. Henneke DR, Potter GD, Kreider JL, Yeates BF. Relationship between condition score, physical measurements and body fat percentage in mares. Equine Vet J. 1983;15:371–372
  14. Draghia-Akli R, Fiorotto ML, Hill LA, Malone PB, Deaver DR, Schwartz RJ. Myogenic expression of an injectable protease-resistant growth hormone releasing hormone augments long-term growth in pigs. Nature Biotech. 1999;17:1179–1183
  15. Pickett BW, Faulkner LC, Seidel GE, Berndtson WE, Voss JL. 1976. Reproductive physiology of the stallion. VI. Seminal and behavioral characteristics. J Anim Sci. 1976;43:617–618
  16. Blanchard L, Varner DD, Schumaker J, Love CC, Brinsko SP, Rodgers JP. Examination of the stallion for breeding soundness. In: Manual of equine reproduction. 2nd ed.. St. Louis: Mosby; 2003;p. 143
  17. Thompson DL, Godke RA, Squires EL. Testosterone effects on mares during synchronization with altrenogest: FSH, LH, estrous duration and pregnancy rate. J Anim Sci. 1983;56:678–686
  18. Thompson DL, Reville SI, Walker MP, Derrick DJ, Papkoff H. 1983. Testosterone administration to mares during estrus: Duration of estrus and diestrus and concentrations of LH and FSH in plasma. J Anim Sci. 1983;56:911–918
  19. Storer WA, Thompson DL, Cartmill JA. The effects of equine somatotropin on pituitary and testicular function in the stallion during the nonbreeding season. J Equine Vet Sci. 2005;25:106–112
  20. Draghia-Akli R, Khan AS, Cummings KK, Parghi D, Carpenter RH, Brown PA. Electrical enhancement of formulated plasmid delivery in animals. Tech Cancer Res Treat. 2002;1:365–372
  21. Gronevik E, von Steyern FV, Kalhovde JM, Tjelle TE, Mathiesen I. Gene expression and immune response kinetics using electroporation-mediated DNA delivery to muscle. J Gene Med. 2005;7:218–227
  22. Khan AS, Smith LC, Abruzzese RV, Cummings KK, Pope MA, Brown PA, et al. Optimization of electroporation parameters for the intramuscular delivery of plasmids in pigs. DNA Cell Biol. 2003;22:807–814
  23. Knobil E. The hypothalamic gonadotrophic hormone releasing hormone (GnRH) pulse generator in the rhesus monkey and its neuroendocrine control. Hum Reprod. 1988;3:29–31
  24. Nishihara M, Takeuchi Y, Tanaka T, Mori Y. Electrophysiological correlates of pulsatile and surge gonadotrophin secretion. Rev Reprod. 1999;4:110–116
  25. Thompson DL, Pickett BW, Squires EL, Nett TM. Effect of testosterone and estradiol-17β alone and in combination on LH and FSH concentrations in blood serum and pituitary of geldings and in serum after administration of GnRH. Biol Reprod. 1979;21:1231–1237
  26. Gentry LR, Thompson DL, Stelzer AM. Responses of seasonally anovulatory mares to daily administration of thyrotropin-releasing hormone and(or) gonadotropin-releasing hormone analogue. J Anim Sci. 2002;80:208–213

 Refereed

 Approved for publication by the Director of the Louisiana Agric. Exp. Sta. as manuscript no. 07-18-0360. We thank A.F. Parlow and the National Institiute of Diabetes and Digestive and Kidney Diseases, National Hormone and Pituitary Program, Harbor-University of California Los Angeles Medical Center, Torrance, CA, and T.G. Ramsay, Growth Biology Laboratory, ARS, USDA, Beltsville, MD, for reagents.

PII: S0737-0806(08)00029-4

doi: 10.1016/j.jevs.2008.01.018

Journal of Equine Veterinary Science
Volume 28, Issue 3 , Pages 149-155 , March 2008

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