Journal of Equine Veterinary Science
Volume 27, Issue 4 , Pages 171-175 , April 2007

Influence of Semen Storage and Cryoprotectant on Post-thaw Viability and Fertility of Stallion Spermatozoa

References 

  1. Crockett EC, Graham JK, Bruemmer JE, Squires EL. Effect of cooling equine spermatozoa before freezing on post-thaw motility: preliminary results. Theriogenology. 2001;57:793–803
  2. Backman T, Bruemmer JE, Graham JK, Squires EL. Pregnancy rates of mares inseminated with semen cooled for 18 hours and then frozen. J Anim Sci. 2004;82:690–694
  3. Squires EL, Pickett BW, Graham JK, Vanderwall DK, McCue PM, Bruemmer JE. Cooled and frozen stallion semen. Animal Reproduction and Biotechnology Laboratory Bulletin No. 9. Colorado State University; 1999;
  4. Douglas-Hamilton DH, Burns PJ, Driscoll DD, Viale KM. Fertility and characteristics of slow-cooled stallion semen. J Reprod Fertil, Suppl. 1987;35:649–650
  5. Squires EL, Brubaker JK, McCue PM, Pickett BW. Effect of sperm number and frequency of insemination on fertility of mares inseminated with cooled semen. Theriogenology. 1998;28:709–718
  6. Varner DD, Blanchard TL, Meyers PJ, Meyers SA. Fertilizing capacity of equine spermatozoa stored for 24 hr at 5°C or 20°C. Theriogenology. 1989;32:515–522
  7. Brinsko SP, Varner DD. Artificial insemination. In: Equine reproduction. Philadelphia: Lea & Febiger; 1992;p. 790–797
  8. Alvarenga MA, Papa FO, Landim-Alvarenga FC, Medeiros ASL. Amides as cryoprotectants for freezing stallion semen: a review. Anim Reprod Sci. 2005;89:105–113
  9. Medeiros ASL, Gomes GM, Carmo MTC, Papa FO, Alvarenga MA. Cryopreservation of stallion sperm using different amides. Theriogenology. 2002;58:273–276
  10. Rossi TC, Papa FO, Santos TB, Macedo LP, Alvarenga MA, Melo CM, et al. Efeito da utilização de diferentes crioprotetores e suas associações no processo de congelação de sêmen eqüino com meio MP50. Rev Bras Reprod Anim. 2003;27:350–352
  11. Zahn FS, Papa FO, Dell'Aqua JA. Cholesterol incorporation on equine sperm membrane: effects on post-thaw sperm parameters and fertility. Theriogenology. 2002;58:237–240
  12. Papa FO, Zahn FS, Dell'Aqua JA, Alvarenga MA. Utilização do diluente MP50 para a criopreservação do sêmen eqüino. Rev Bras Reprod Anim. 2002;26:184–187
  13. Dell'Aqua JA, Papa FO, Zahn FS. Effects of warming rate on sperm parameters and of insemination site and dose on the fertility of equine frozen semen. Anim Reprod Sci. 2001;68:344–346
  14. Harrison RA, Vickers SE. Use of fluorescent probes to assess membrane integrity in mammalian spermatozoa. J Reprod Fertil. 1990;88:343–352
  15. Zúccari CESN. Efeito da criopreservação sobre a integridade estrutural da célula espermática eqüina. PhD Thesis, Faculdade de Medicina Veterinária e Zootecnia. Universidade Estadual Paulista. 1998;121 pp
  16. Batellier F, Vidament M, Fauquant J, Duchamp G, Arnaud G, Yvon JM, et al. Advances in cooled semen technology. Anim Reprod Sci. 2001;68:181–190
  17. Amann RP, Pickett BW. Principles of cryopreservation and a review of cryopreservation of stallion spermatozoa. J Equine Vet Sci. 1987;7:145–173
  18. Fahy GM. The relevance of cryoprotectant toxicity to cryobiology. Cryobiology. 1986;23:1–13
  19. Gomes GM, Jacob JCF, Medeiros ASL, Papa FO, Alvarenga MA. Improvement of stallion spermatozoa preservation with alternative cryoprotectants for Mangalarga Marchador breed. Theriogenology. 2002;58:277–279
  20. Squires EL, Keith SL, Graham JK. Evaluation of alternative cryoprotectants for preserving stallion spermatozoa. Theriogenology. 2004;62:1056–1065
  21. Samper JC, Morris CA. Current methods for stallion semen cryopreservation: a survey. Theriogenology. 1998;49:895–903
  22. Neves Neto JR. Avaliação morfo-funcional e fertilidade de sêmen congelado eqüino utilizando diferentes diluentes e substâncias crioprotetoras. PhD Thesis, São Paulo State University, Botucatu, Brazil; 2004.
  23. Dell'Aqua JA Jr. Efeito da centrifugação, tipos de envase e temperatura de descongelação sobre os parâmetros espermáticos e índices de fertilidade relacionados com o local de deposição e concentração da dose inseminante do sêmen congelado eqüino. MS Thesis, São Paulo State University, Botucatu, Brazil; 2000.
  24. Leão KM. Inseminação artificial por endoscopia com número reduzido de espermatozóides utilizando sêmen fresco e congelado de garanhões. MS Thesis, São Paulo State University, Botucatu, Brazil; 2002.

 Refereed

PII: S0737-0806(07)00085-8

doi: 10.1016/j.jevs.2007.02.008

Journal of Equine Veterinary Science
Volume 27, Issue 4 , Pages 171-175 , April 2007

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