Journal of Equine Veterinary Science
Volume 27, Issue 1 , Pages 14-19 , January 2007

Molecular Monitoring of the Main Changes in Bacterial Floral Diversity in the Gastrointestinal Tract of a Thoroughbred Foal with Catarrhal Enteritis by Using PCR-DGGE

  • Hidetoshi Morita, PhD

      Affiliations

    • From the School of Veterinary Medicine, Azabu University, Kanagawa, Japan
    • Corresponding Author InformationReprint requests: Hidetoshi Morita, School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Sagamihara, Kanagawa 229-8501, Japan.
    • ,
  • Fumihiko Nakajima, DVM

      Affiliations

    • Northern Farm, Hokkaido, Japan
    • ,
  • Masaru Murakami, DVM, PhD

      Affiliations

    • From the School of Veterinary Medicine, Azabu University, Kanagawa, Japan
    • ,
  • Akihito Endo, PhD

      Affiliations

    • NODAI Culture Collection Center, Tokyo University of Agriculture, Tokyo, Japan
    • ,
  • Takehito Suzuki, DVM, PhD

      Affiliations

    • From the School of Veterinary Medicine, Azabu University, Kanagawa, Japan
    • ,
  • Chiharu Shiratori (bachelor)

      Affiliations

    • From the School of Veterinary Medicine, Azabu University, Kanagawa, Japan
    • ,
  • Yukio Kato, DVM, PhD

      Affiliations

    • From the School of Veterinary Medicine, Azabu University, Kanagawa, Japan
    • ,
  • Alexandre T. Okatani, DVM, PhD

      Affiliations

    • From the School of Veterinary Medicine, Azabu University, Kanagawa, Japan
    • ,
  • Hiroaki Akita, DVM, PhD

      Affiliations

    • Northern Farm, Hokkaido, Japan
    • ,
  • Toshio Masaoka, DVM, PhD

      Affiliations

    • From the School of Veterinary Medicine, Azabu University, Kanagawa, Japan

References 

  1. Millar MR, Linton CJ, Cade A, Glancy D, Hall M, Jalal H. Application of 16S RNA gene PCR to study bowel flora of preterm infants with and without necrotizing enterocolitis. J Clin Microbiol. 1996;34:2506–2510
  2. Christine FF, Vaughan EE, de Vos WM, Akkermans AD. Molecular monitoring of succession of bacterial communities in human neonates. Appl Environ Microbiol. 2002;68:219–226
  3. Siqueira JF, Rocas IN, Rosado AS. Application of denaturing gradient gel electrophoresis (DGGE) to the analysis of endodontic infections. J Endod. 2005;31:775–782
  4. Peloso JG, Mundy GD, Cohen ND. Prevalence of, and factors associated with, musculoskeletal racing injuries of thoroughbreds. J Am Vet Med Assoc. 1994;204:620–626
  5. Perrin J, Cosmetatos I, Gallusser A, Lobsiger L, Straub R, Nicolet J. Clostridium difficile associated with typhlocolitis in an adult horse. J Vet Diagn Invest. 1993;5:99–101
  6. Netherwood T, Wood JL, Townsend HG, Mumford JA, Chanter N. Foal diarrhoea between 1991 and 1994 in the United Kingdom associated with Clostridium perfringens, rotavirus, Strongyloides westeri and Cryptosporidium spp. Epidemiol Infect. 1996;117:375–383
  7. Matsuki T, Watanabe K, Fujimoto J, Miyamoto Y, Takada T, Matsumoto K, et al. Development of 16S rRNA-gene-targeted group-specific primers for the detection and identification of predominant bacteria in human feces. Appl Environ Microbiol. 2002;68:5445–5451
  8. Muyzer G, de Waal E, Uitterlinden A. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl Environ Microbiol. 1993;59:695–700
  9. Morita H, Yoshikawa H, Suzuki T, Hisamatsu S, Kato Y, Sakata R, et al. Anti-microbial action against verotoxigenic Escherichia coli O157:H7 of nitric oxide derived from sodium nitrite. Biosci Biotechnol Biochem. 2004;68:1027–1034
  10. Pridmore RD, Berger B, Desiere F, Vilanova D. The genome sequence of the probiotic intestinal bacterium Lactobacillus johnsonii NCC 533. Proc Natl Acad Sci U S A. 2004;101:2512–2517
  11. Paulsen IT, Banerjei L, Myers GS, Nelson KE, Seshadri R, Read TD, et al. Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus faecalis. Science. 2003;299:2071–2074
  12. Beutin L, Geier D, Steinrück H, Zimmermann S, Scheutz F. Prevalence and some properties of verotoxin (Shiga-like toxin)-producing Escherichia coli in seven different species of healthy domestic animals. J Clin Microbiol. 1993;31:2483–2488
  13. Heuvelink AE, van den Biggelaar FL, Zwartkruis-Nahuis J, Herbes RG, Huyben R, Nagelkerke N, et al. Occurrence of verocytotoxin-producing Escherichia coli O157 on Dutch dairy farms. J Clin Microbiol. 1998;36:3480–3487
  14. Chalmers RM, Salmon RL, Willshaw GA, Cheasty T, Looker N, Davies I, et al. Vero-cytotoxin-producing Escherichia coli O157 in a farmer handling horses. The Lancet. 1997;349:1816

 Refereed

PII: S0737-0806(06)00634-4

doi: 10.1016/j.jevs.2006.11.011

Journal of Equine Veterinary Science
Volume 27, Issue 1 , Pages 14-19 , January 2007

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