Journal of Equine Veterinary Science
Volume 26, Issue 11 , Pages 529-534 , November 2006

Effect of embryo age on the viability of equine embryos after cooled storage using two transport systems

  • M. Moussa, DVM, PhD

      Affiliations

    • UMR INRA-CNRS-Univ. Tours-Haras Nationaux “PRC” 37380, Nouzilly France
    • Laboratory of Biotechnology and Pathology of Reproduction, National Veterinary School of Nantes, BP 40706, 44307 Nantes, France
    • Al-Baath University, Faculty of Veterinary Medicine, Hama, Syria
    • ,
  • G. Duchamp

      Affiliations

    • UMR INRA-CNRS-Univ. Tours-Haras Nationaux “PRC” 37380, Nouzilly France
    • ,
  • P.F. Daels, DVM, PhD, Dipl ACT ECAR

      Affiliations

    • UMR INRA-CNRS-Univ. Tours-Haras Nationaux “PRC” 37380, Nouzilly France
    • ,
  • J-F. Bruyas, DVM, PhD, Dipl ECAR

      Affiliations

    • Laboratory of Biotechnology and Pathology of Reproduction, National Veterinary School of Nantes, BP 40706, 44307 Nantes, France
    • Corresponding Author InformationReprint requests: J.F. Bruyas, ENVN, BP40706, Nantes Cedex 44307, France

References 

  1. Squires EL , McCue PM , Vanderwall DK . The current status of equine embryo transfer . Theriogenology . 1999;51:91–104
  2. Carnevale EM , Squires EL , McKinnon AO . Comparison of Ham's F-10 with CO2 or Hepes buffer for storage of equine embryos at 5°C for 24h . J Anim Sci . 1987;65:1775–1781
  3. Good NE , Winget GD , Winter W , Connolly TN , Izawa S , Singh RMM . Hydrogen ion buffers for biological research . Biochemistry . 1966;5:467–477
  4. Seshagiri PB , Bavister BD . Glucose and phosphate inhibit respiration and oxidative metabolism in cultured hamster eight-cell embryos: evidence for the “crabtree effect.” . Mol Reprod Dev . 1991;30:105–111
  5. McCue PM , Scoggin CF , Meira C , Squires EL . Pregnancy rates for equine embryos cooled for 24 hours in Ham's F-10 vs Emcare™ embryo holding solution . Proceedings of the Society for Theriogenology . 2000;147; (abstr).
  6. Moussa M , Duchamp G , Mahla R , Bruyas J-F , Daels PF . In vitro and in vivo comparison of Ham's F10, Emcare Holding Solution and ViGro Holding Plus for the cooled storage of equine embryos . Theriogenology . 2003;59:1615–1625
  7. Barros CW , Bényei B . Comparison between zwitterion and phosphate buffer-based bovine embryo handling solutions for embryo storage and transfer in a tropical environment . Theriogenology . 2000;53:308; (abstr).
  8. Nelson LD , Nelson CF . Handling and culture of bovine embryos: survey of media used by 26 embryos transfer companies in the USA . Theriogenology . 2001;56:1377–1382
  9. Squires EL , Imel KJ , Iuliano MF , Shideler RK . Factors affecting reproductive efficiency in an equine embryo transfer programme . J Reprod Fertil . 1982;32(Suppl):409
  10. Iuliano MF , Squires EL , Cook VM . Effect of age of equine embryos and method of transfer on pregnancy rate . J Anim Sci . 1985;60:258–263
  11. Lindner GM , Anderson GB , BonDurant RH , Cupps PT . Survival of bovine embryos stored at 4°C . Theriogenology . 1983;20:311–319
  12. Clark KE , Squires EL , McKinnon AO , Seidel GE . Viability of stored equines embryos . J Anim Sci . 1987;65:534–542
  13. Fleury JJ , Fleury PDC , Landim-Alvarenga FC . Effect of embryo diameter and storage period on pregnancy rates obtained with equine embryos stored in Ham's F-10 with Hepes buffer at a temperature of 15–18°C preliminary results . Theriogenology . 2002;58:749–750
  14. Moussa M , Tremoleda JL , Duchamp G , Bruyas J-F , Colenbrander B , Bevers MM , et al.   Evaluation of viability and apoptosis on horse embryos stored under different conditions at 5°C . Theriogenology . 2004;61:921–932
  15. Duchamp G , Bour B , Combarnous Y , Palmer E . Alternative solutions to hCG induction of ovulation in the mare . J Reprod Fertil . 1987;35(Suppl):221–228
  16. McKinnon AO , Squires EL . Morphologic assessment of the equine embryo . J Am Vet Med Assoc . 1988;192:401–406
  17. Lagneaux D , Duchamp G , Bruneau B , Pommarici AM , Risco F , Peltier M , et al.   Le transport d'embryons équins réfrigérés: mise en place et premier bilan . J Recherche Equine . 2006;26:131–135
  18. Moussa M, Duchamp G, Bruyas J-F, Daels PF. Correlation between the number of cells and the diameter of equine embryos between 6.5 and 8 days. In: Proceedings of the 20th Scientific Meeting of the European Embryo Transfer Association; 2004:160 (abstr).
  19. Ginther OJ . In: Reproductive biology of the mare: basic and applied aspects . 2nd Ed.. Cross Plains, WI: EquiServices; 1992;p. 299–300 499-545.
  20. Carnevale EM , Ramirez RJ , Squires EL , Alvarenga MA , Vanderwall DK , McCue PM . Factors affecting pregnancy rates and early embryonic death after equine embryo transfer . Theriogenology . 2000;54:965–979

 Refereed

PII: S0737-0806(06)00500-4

doi: 10.1016/j.jevs.2006.09.009

Journal of Equine Veterinary Science
Volume 26, Issue 11 , Pages 529-534 , November 2006

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