Journal of Equine Veterinary Science
Volume 26, Issue 9 , Pages 406-411 , September 2006

Effect of pyruvate and lactate on motility of cold stored stallion spermatozoa challenged by hydrogen peroxide

  • Gary W. Webb, PhD, PAS

      Affiliations

    • Department of Agriculture, Missouri State University, Springfield, MO
    • Corresponding Author InformationReprint requests: Gary W. Webb, PhD, PAS, Department of Agriculture, 901 South National, Missouri State University, Springfield, MO 65897
    • ,
  • Mark J. Arns, PhD, PAS

      Affiliations

    • Department of Agriculture, Missouri State University, Springfield, MO

References 

  1. Todd P , Arns MJ , Chenoweth P , Schultz B . Influence of seminal plasma and processing on cold stored stallion spermatozoa . Anim Reprod Sci . 2001;68:335–336 (abstr)
  2. Jasko DJ , Moran DM , Farlin ME , Squires EL . Effect of seminal plasma dilution or removal on spermatozoal motion characteristics of cooled stallion semen . Theriogenology . 1991;35:1059–1067
  3. Dawson GR , Webb GW , Pruitt JA , Loughlin TM , Arns MJ . Effect of different processing techniques on motility and acrosomal integrity of cold-stored stallion spermatozoa . J Equine Vet Sci . 2000;20:191–194
  4. Brinsko SP , Crockett EC , Squires EL . Effect of centrifugation and partial removal of seminal plasma on equine spermatozoal motility after cooling and storage . Theriogenology . 2000;54:129–136
  5. Padilla AW , Foote RH . Extender and centrifugation effects on the motility patterns of slow-cooled stallion spermatozoa . J Anim Sci . 1991;69:3308–3313
  6. Webb GW , Arns MJ . Influence of modified Tyrode's media on motility of cold-stored stallion spermatozoa . J Equine Vet Sci . 1994;15:441–444
  7. Rigby SL , Brinsko SP , Cochran M , Blanchard TL , Love CC , Varner DD . Advances in cooled semen technologies: seminal plasma and semen extender . Anim Reprod Sci . 2001;68:171–180
  8. Webb GW , Arns MJ . Influence of Na+/K+ ratios on cold-stored equine spermatozoa . Theriogenology . 1996;45:312
  9. Webb GW, Arns MJ. Effect of different salt solutions on the motility of cold-stored equine spermatozoa. Proc 15th Equine Nutr Physiol Symp; 1997.
  10. Baumber J , Vo A , Sabeur K , Ball BA . Generation of reactive oxygen species by equine neutrophils and their effect on motility of equine spermatozoa . Theriogenology . 2002;57:1025–1033
  11. Bilodeau JF , Blanchette S , Cormier N , Sirard MA . Reactive oxygen species-mediated loss of bovine sperm motility in egg yolk Tris extender: protection by pyruvate, metal chelators and bovine liver or oviductal fluid catalase . Theriogenology . 2002;57:1105–1122
  12. Armstrong JS , Rajasekaran M , Chamulitrat W , Gatti P , Hellstrom WJ , Sikka SC . Characterization of reactive oxygen species induced effects on human spermatozoa movement and energy metabolism . Free Radic Biol Med . 1999;26:869–880
  13. Cavinder CA , Harrison K , Webb GW . Antioxidant effects of pyruvate as a component of extenders for stallion semen . Proc Equine Nutr Physiol Soc Symp; . 2003;262–263
  14. Bruemmer JE , Coy RC , Squires EL , Graham JK . Effect of pyruvate on the function of stallion spermatozoa stored for up to 48 hours . J Anim Sci . 2002;80:12–18
  15. Neild DM , Gadella BM , Chaves MG , Miragaya MH , Colenbrander B , Agüero A . Membrane changes during different stages of a freeze-thaw protocol for equine semen cryopreservation . Theriogenology . 2003;59:1693–1705
  16. Ball BA , Medina V , Gravance CG , Baumber J . Effect of antioxidants on preservation of motility, viability and acrosomal integrity of equine spermatozoa during storage at 5°C . Theriogenology . 2001;56:577–589
  17. Paulson JD , Polaskoski KL . Isolation of a spermatozoal immobilization factor from Escherichia coli filtrates . Fertil Steril . 1997;28:182–185
  18. Gladden LB . Lactate metabolism: a paradigm for the third millennium . J Physiol . 2003;558:5–30
  19. Jones AR . Metabolism of lactate by mature boar spermatozoa . Reprod Fertil Dev . 1997;9:227–232
  20. Pesch S , Bergmann M , Bostedt H . Determination of some enzymes and macro- and microelements in stallion seminal plasma and their correlations to semen quality . Theriogenology . 2006;66:307–313
  21. Aurich C . Factors affecting the plasma membrane function of cooled-stored stallion spermatozoa . Anim Reprod Sci . 2005;89:65–75
  22. Kankofer M , Kolm G , Aurich JE , Aurich C . Activity of glutathione peroxidase, superoxide dismutase and catalase and lipid peroxidation intensity in stallion semen during storage at 5°C . Theriogenology . 2005;63:1354–1365

 Refereed

PII: S0737-0806(06)00393-5

doi: 10.1016/j.jevs.2006.07.006

Journal of Equine Veterinary Science
Volume 26, Issue 9 , Pages 406-411 , September 2006

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