Journal of Equine Veterinary Science
Volume 26, Issue 7 , Pages 305-309 , July 2006

Retention of liquid within insemination equipment using various equine frozen semen insemination methods and two semen freezing extenders

  • John J. Dascanio, VMD, Diplomate ACT, ABVP

      Affiliations

    • Virginia-Maryland Regional College of Veterinary Medicine, Department of Large Animal Clinical Sciences, Center for Reproductive Excellence using Assisted Reproduction and Endocrinology (CREATE)
    • Corresponding Author InformationReprint requests: John J. Dascanio, Virginia-Maryland Regional College of Veterinary Medicine, Department of Large Animal Clinical Sciences, Center for Reproductive Excellence using Assisted Reproduction and Endocrinology (CREATE), Duck Pond Drive, Virginia Tech, Blacksburg, VA 24061
    • ,
  • Ramanathan Kasimanickam, BVSc, DVSc, Diplomate ACT

      Affiliations

    • Virginia-Maryland Regional College of Veterinary Medicine, Department of Large Animal Clinical Sciences, Center for Reproductive Excellence using Assisted Reproduction and Endocrinology (CREATE)
    • ,
  • Stephanie Milburn, BS

      Affiliations

    • Virginia-Maryland Regional College of Veterinary Medicine, Department of Large Animal Clinical Sciences, Center for Reproductive Excellence using Assisted Reproduction and Endocrinology (CREATE)
    • ,
  • Andrea Canerossi, BS

      Affiliations

    • Department of Animal and Poultry Sciences, Virginia Tech, Blacksburg, VA
    • ,
  • Rebecca Splan, PhD

      Affiliations

    • Department of Animal and Poultry Sciences, Virginia Tech, Blacksburg, VA

References 

  1. Leipold SD , Graham JK , Squires EL , McCue PM , Brinsko SP , Vanderwall DK . Effect of spermatozoal concentration and number on fertility of frozen equine semen . Theriogenology . 1998;49:1537–1543
  2. Graham JK . Procedures for cryopreserving sperm: sperm packaging, freeze/thaw rates and method of freezing . Proc Annual Mtg Soc Theriogenology . 1999;259–266
  3. Heitland AV , Jasko DJ , Squires EL , Graham JK , Pickett BW , Hamilton C . Factors affecting motion characteristics of frozen-thawed stallion spermatozoa . Equine Vet J . 1996;28:47–53
  4. Loomis PR , Amann RP , Squires EL , Pickett BW . Fertility of unfrozen and frozen stallion spermatozoa extended in EDTA-lactose-egg yolk and packaged in straws . J Anim Sci . 1983;56:687–693
  5. Loomis PR , Clark JS . Motion characteristics of frozen-thawed equine spermatozoa packaged in 0.5ml straws at various concentrations . Proc Annual Mtg Soc Theriogenology . 1998;142
  6. Martin JC , Klug E , Gunzel AR . Centrifugation of stallion semen and its storage in large volume straws . J Reprod Fertil (Suppl) . 1979;27:47–51
  7. Sieme H , Bonk A , Hamann H , Klug E , Katila T . Effects of different artificial insemination techniques and sperm doses on fertility of normal mares and mares with abnormal reproductive history . Theriogenology . 2004;62:915–928
  8. Slusher SH , Freeman KP , Roszel JF . Eosinophils in equine uterine cytology and histology specimens . J Am Vet Med Assoc . 1984;184:665–670

 Refereed

PII: S0737-0806(06)00299-1

doi: 10.1016/j.jevs.2006.05.002

Journal of Equine Veterinary Science
Volume 26, Issue 7 , Pages 305-309 , July 2006

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